The use of the Cas9 vector pDCC6 to mutate the dTRC8 gene in Drosophila melangoster

Elle Choate

Gene editing technologies have become more widely used and highly effective in recent years with the progression of research. CRISPR/Cas is a system that allows researchers to target a specific gene for mutation. In this project, our goal is to mutate the dTRC8 gene in Drosophila melanogaster using the CRISPR method of gene editing. We have found an ideal plasmid called pDCC6 as it contains the Cas vector and sgRNA scaffold necessary. Appropriate DNA sequences were designed, which will then be ligated into the vector, and transformed using E. coli. After being transformed the plasmid will be purified and sequenced to verify that it is correct. After verification, this vector can be used in cultured cells or used to make transgenic animals.

• Elle Choate is a junior majoring in biology with an emphasis in genetics and is planning on transferring to Clemson in the fall of 2021 to pursue an undergraduate and master’s degree in bioengineering while completing her degree at Lander. At Clemson, she plans to pursue research in genetic engineering or prosthetics.