Unveiling SFK Family Expression in Zebrafish Organs: Exploring BLK Modulation and its Effects on Embryonic Development

Rida Hirani

SRC Family Kinases (SFKs) are proteins found in cells that regulate the growth and mobility of cells. Specific components of SRC kinase bind to the receptors to create complexes that are essential for cell signaling. Dysregulation of SFKs is a common route for cancer development. Our study started with SFK family members, LYN, BLK, and SRC. Our research began by dissecting the adult zebrafish organs and extracting the RNA from adult tissues. To investigate the potential expression among SFK family members, the gene expression of these SFK variants was compared to a housekeeping gene Actin, to determine if there is any difference in expression. Future research studies will focus on modulation of BLK expression. BLK mRNA will be microinjected into zebrafish embryos to assess the impact of overexpression in cells. The development of embryos will be observed, and any effects or modifications to the organ systems' development will be assessed.

• Rida Hirani, who graduated from Greenwood High School, is pursuing a major in medical biology. She actively engages in on-campus activities, having received the New Student Leader Award in 2023 and the Hall of Leaders Award. Rida's articles have been published three times by Lander’s New Voices. Furthermore, she participates in the Honors College Leadership Council, serves as an officer for Lander HOSA, is involved in the Student Alumni Association, holds the position of Vice President in Lander ESSO, and serves as a senator in the Student Government Association.